FastQCFastQC Report
Mon 27 Feb 2023
SRR3128912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371420
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307311.295890226109251No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG34750.14653667422894298No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC30010.126548650175844TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119800.084.662051
ATAGGGC14300.072.303923
TAGGGCA11500.071.9266744
AGGGATG36850.071.420975
AGTAGGG35600.069.8848652
AGTAAGG17250.069.574381
GGTAAGG14300.068.458211
TATAGGG18550.068.453762
ATAGGGA18700.068.360063
TAGGGAT12800.067.926144
GTAAGGG39200.067.1861042
TAAGGGA18650.067.031353
GAATAGG15700.066.850321
AATAGGG33550.066.4451452
TAGAGGG43350.066.0702062
ACGGGTA2000.065.796564
CGTAGGG5800.065.68022
GAGGGAT33200.065.5417564
AAGGGAT36150.065.393544
AAGGGGC78700.064.912744
Produced by FastQC (version 0.11.5)