FastQCFastQC Report
Mon 27 Feb 2023
SRR3128911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491958
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319451.281923692132853No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTG37020.1485578809915737No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC31520.1264868830052513TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT123050.084.376981
ACGGGTA1650.071.2071154
ATAGGGC14550.071.06033
AGTAGGG40750.069.658912
ATAGGGA20150.068.80413
AGGGATG38550.068.0222555
AGTAAGG18500.067.387211
AGATAGG15050.066.58041
TATAGGG18100.066.2108152
TAAGGGA19500.066.0363853
GTAAGGG41000.066.024632
AAGGGGC86600.065.71944
TAGAGGG46450.065.4614942
ATAGCGG4550.065.13771
AAGGGAT38100.064.759234
ATAAGGG33100.064.318752
GGTAAGG14750.064.1072851
TAGGGCA13150.063.61534
AGGGCAT19750.063.5309525
AATAGGG35800.063.1436122
Produced by FastQC (version 0.11.5)