FastQCFastQC Report
Mon 27 Feb 2023
SRR3128910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253026
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162530.7213853723836299No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC44970.1995982292259388Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG34560.15339370251386358No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG31010.1376371155947601No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28420.1261414648565973No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.10501432295943322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA2650.081.887274
CGTTTTT59000.078.3050841
CGTAGGG6350.075.7758562
AGGGATG35000.075.455765
GTAGGGT7650.075.232033
GAATAGG15300.073.408231
ATAGGGA22300.072.347853
AGGGTAT13750.071.427875
TAGGGCA11700.071.366074
TATAGGG17250.071.103122
GTAGGGC8900.071.0263063
TAGGGTA6900.070.419434
GAGGGAT28850.069.821064
ATAGGGC14000.069.413453
AAGGGTA16100.068.563724
CGAAGGG22650.068.522322
TAGAGGG39300.068.420572
AGTAGGG33900.067.073622
AAGGGAT37400.066.472724
ATAAGGG26050.066.4603652
Produced by FastQC (version 0.11.5)