FastQCFastQC Report
Mon 27 Feb 2023
SRR3128909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317763
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158420.6835038785242494No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC48290.20834744536003033Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG36060.15558104948607776No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG33030.14250809940446887No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31020.13383594439983726No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62000.078.681551
ATAACGG2050.078.02091
AGGGATG36400.073.722295
ATAGGGC14650.073.141063
CGTAGGG6700.072.979462
ACGGGTA3100.072.768534
GAATAGG16300.072.727531
TAGGGCA11400.072.143514
GAGGGAT30350.071.6945954
AGGGTAC12400.070.4945145
TAGGGTA7300.068.8850564
GTAGGGT8250.068.3583153
AGGGTAT14050.067.902185
TATAGGG17450.067.896482
GAGGGTA12550.067.405114
GTAGGGC9350.067.353053
ATAAGGG28700.067.328982
TAGAGGG40800.066.835922
ATAGAGG15750.066.6055151
ATAGGGA22850.066.4324653
Produced by FastQC (version 0.11.5)