FastQCFastQC Report
Mon 27 Feb 2023
SRR3128908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430540
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162210.6673825569626503No Hit
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC49410.20328815818706955Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG37200.15305240810684045No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTG35800.14729237124260453No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31410.12923054136117898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61850.078.714351
AGGGATG36050.072.087835
ACGGGTA3000.072.061864
CGTAGGG6150.071.83262
ATAGGGC14650.071.217093
TAGGGCA13500.070.3212364
TAGGGTA6900.070.154734
TAGAGGG44450.069.147242
GAATAGG15650.069.130031
AGTACGG3000.068.989761
ATAGGGA22500.068.928733
AGGGTAC12700.068.8258065
GTAGGGT9800.068.577063
AAGGGTA17600.067.557994
GAGGGAT29850.067.543234
AGGGTAT15750.067.134235
CGAAGGG25400.066.9797742
ACGGGAT7900.066.628474
GAGGGTA15100.066.293594
ATAGAGG16250.065.99861
Produced by FastQC (version 0.11.5)