FastQCFastQC Report
Mon 27 Feb 2023
SRR3128907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415487
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194770.8063384319600976No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG25740.10656236195847876No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC25570.10585857013513218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81900.082.655451
GGTAAGG15500.070.6089551
GAATAGG14500.068.971771
AGGGTAC11050.067.619515
ATAGGGC14050.067.4636463
AGTAAGG15400.067.085231
AGGGAAT22000.067.072445
GTACGGG6750.067.068342
TAGGGCA12450.067.043094
AGGGATG34700.066.9013065
AGTAGGG38400.066.806362
AGAGGGC36750.066.084563
AAGGGTA14500.066.02044
ATAGGGA18300.065.711173
AGGGCAT20650.065.540475
AATAGGG38150.065.019152
ATAAGGG33950.065.006412
TATAGGG17450.064.858352
AAGAGGG85600.064.786372
GTAAGGG39250.064.758862
Produced by FastQC (version 0.11.5)