FastQCFastQC Report
Mon 27 Feb 2023
SRR3128906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451126
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186590.7612419761366817No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC26740.10909271901974847No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG25920.10574731776334632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT77850.081.366871
ATAGGGC13100.074.260413
ATAGGGA19000.071.235613
AGTAAGG16400.069.730631
TAGGGCA11350.068.319764
GGATAGG17600.068.184971
TATAGGG17500.067.713892
AGGGATG35300.067.631235
GGTAAGG16600.067.4731
AGGGAAT22550.067.315375
AGGGTAC11550.067.136745
AGTAGGG35600.066.836822
CGTAGGG7250.066.805852
AAGGGCG15200.066.783394
AAGGGTA15800.066.32944
GAATAGG14850.065.917081
GATAGGG38450.065.429372
TAGAGGG44800.065.1821752
ATAGAGG15950.064.911721
AAGGGAT39250.064.8959964
Produced by FastQC (version 0.11.5)