FastQCFastQC Report
Mon 27 Feb 2023
SRR3128905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578060
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199290.7730231259163867No Hit
CTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTGC27440.10643662288697703No Hit
CCTGTCTCTTATACACATCTGACGCGAATCACGTCGTATGCCGTCTTCTG26740.10372140291537046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78450.081.608621
GAATAGG14950.072.684461
AGGGATG36050.070.1363755
TAGGGCA12450.069.455464
ATAGGGA21100.068.156183
TATAGGG19150.067.730042
AGTAGGG36350.067.226422
TAGAGGG48250.066.814062
GGTAAGG16500.066.426721
CGTAGGG7300.066.30642
ATAGGGC16950.066.266573
AAGGGAT43950.065.86884
AGTAAGG17150.065.829111
AATAGGG39000.065.67092
GTACGGG6950.065.588542
AAGGGTA17450.065.443834
AGGGAAT23100.065.3069465
AGGGTAC12100.065.251465
ATAAGGG37200.064.300732
TAGGGTA6800.064.273494
Produced by FastQC (version 0.11.5)