FastQCFastQC Report
Mon 27 Feb 2023
SRR3128904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642040
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184810.6994973581020726No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC46490.1759625138150823No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG46260.17509197438343096No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG39420.14920288867693146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72500.082.101841
AGGGATG48300.075.694895
CGTAGGG7700.074.0930942
ATAGGGC14500.073.48923
TAAGGGA23000.072.160233
ATAGCGG5500.072.035761
GTAGGGC13900.071.912463
AGTAGGG42600.071.832042
AAGGGTA18850.071.788294
AGGGTAC14950.071.668415
GAGGGAT38750.071.546554
TAGGGCA14050.070.809134
AAGGGAT56400.070.725224
TAGAGGG44800.070.198992
CGAAGGG26300.068.663532
TAGGGAT24650.068.0951464
AGAGGGA65650.067.7985463
GGTAAGG16650.067.704141
AGGGATT36650.066.931515
ATAGGGA27650.066.6752553
Produced by FastQC (version 0.11.5)