FastQCFastQC Report
Mon 27 Feb 2023
SRR3128903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752563
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181830.6605843353994078No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC51790.18815191514236004No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG47230.17158553682513353No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG42800.155491445609056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78450.081.009061
AGGGATG51250.076.205045
ATAGCGG6550.073.2537841
AAGGGAT59050.072.744954
TAGGGTA9150.070.368134
GAGGGAT42250.070.07944
ATGCGGG14050.069.612072
AGGGATT38100.069.324695
AAGGGTA24000.069.3215944
AGGGTAC15600.069.291475
TAAGGGA27450.069.169653
GGTAAGG17400.068.66821
ATAGGGA26900.068.6620563
AGTAGGG48100.068.626132
ATAGGGC16700.068.104443
CGGGATG10300.067.986965
TAGGGCA14550.067.831724
AGGGATC24100.067.2788545
ACGGGAT9100.067.1395654
GTAGGGC12150.066.5317153
Produced by FastQC (version 0.11.5)