FastQCFastQC Report
Mon 27 Feb 2023
SRR3128902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2856629
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183800.6434157183169393No Hit
CTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTGC54990.19249962105684706No Hit
CCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG51780.18126260007862413No Hit
GCTGTCTCTTATACACATCTGACGCTAAGTCCTTCGTATGCCGTCTTCTG44130.1544827837286536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75800.081.501341
AGGGATG50450.075.362375
CGTAGGG7400.071.131362
AAGGGAT58850.070.915524
AAGGGTA21600.070.278684
ATAGCGG6850.069.374531
GGTAAGG20050.069.227211
GAGGGAT42650.068.760714
TAGGGCG5950.068.719054
TAAGGGA28200.068.163053
AGGGTAC15600.068.084945
AGGGATC23350.068.029465
ATGCGGG13900.067.960422
AGTAGGG49150.067.8905942
TAGGGAT25900.067.865124
ATAGGGC15500.067.615763
GTAGGGT9000.067.363093
AGGGTAT15950.067.1802145
AAGAGGG99250.067.09872
ATAGGGA27500.066.9928053
Produced by FastQC (version 0.11.5)