FastQCFastQC Report
Mon 27 Feb 2023
SRR3128901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496124
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212140.8498776503090392No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG31890.12775807612121834No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC30670.12287049842075154Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84150.081.1434561
AGGGATG39700.073.15165
CGTAGGG5700.071.998972
TAGGGCA12150.071.04884
AGGGTAC14700.070.967825
ATAGGGC14450.070.8392263
GAATAGG15250.070.5511551
GTAGGGC10450.069.064843
GGTAAGG17600.068.638291
AAGGGAT39800.068.6242144
AAGGGTA17200.068.289324
ATAGGGA22700.067.952243
TAAGGGA22350.066.6947253
AGTAAGG14900.066.191031
AGAGGGC36550.066.0791553
ATGAGGG40200.066.063912
TAGAGGG47600.065.9016952
AGTAGGG40250.065.630252
AGATAGG13400.065.50091
ATAGAGG17850.065.297691
Produced by FastQC (version 0.11.5)