FastQCFastQC Report
Mon 27 Feb 2023
SRR3128900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551574
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209920.8227078658114559No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG33910.1328983599926947No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC32370.12686286974236294Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84200.080.9120561
CGTAGGG6800.078.829482
AGGGATG41650.074.811775
AGGGTAC13400.071.898615
TAGGGCA12800.070.862884
AGTAGGG39300.069.0360642
GTAGGGC12350.068.8783953
GAGGGTA15450.068.442434
ATAGAGG17600.068.435911
GAGGGAT34550.068.013234
ATAGGGC14600.067.9205253
ATAGGGA21200.067.613813
AAGGGTA15650.067.5677644
GGTAAGG14450.067.07431
TAGGGTA7450.066.8684164
GAATAGG16500.066.725021
AAGGGAT40600.066.212724
GTACGGG6700.065.9698942
ATGAGGG42550.065.531162
AAGAGGG94400.065.351352
Produced by FastQC (version 0.11.5)