FastQCFastQC Report
Mon 27 Feb 2023
SRR3128899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664890
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216350.8118533973259684No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG36380.13651595375418873No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC36060.13531515372116673Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG27050.10150512779139101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86950.079.389771
CGTAGGG5600.077.207152
AGGGATG41850.074.676495
GAATAGG16550.069.373861
AGGGTAC13100.069.237875
ATAGGGC15100.069.0942463
GTAGGGC12450.069.079253
GGTAAGG15800.068.7953341
ATAGGGA21300.068.619363
TAGGGCA13700.068.6080864
TAGAGGG51500.068.349022
ATAGAGG18550.067.221161
AGTAGGG42600.067.073612
AAGGGTA15300.066.655224
AAGGGAT43400.066.055044
ATAAGGG39550.065.9483952
TAAGGGA23550.065.6554643
ATGAGGG42700.065.485752
AATAGGG40050.065.359752
TAGGGTA7700.065.306884
Produced by FastQC (version 0.11.5)