FastQCFastQC Report
Mon 27 Feb 2023
SRR3128898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160776
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153900.712244119705143No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG48380.22390104295864077No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC47380.21927307596900375Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG44370.2053428953301962No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22830.105656486373414No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21750.10065828202460597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5900.076.732362
AGGGATG39450.076.593715
CGTTTTT61500.075.106721
ATAGCGG4350.073.754891
ATAGAGG12200.072.7058261
AGGGTAC9400.072.4885255
AGTAGGG33250.071.6240842
GAGGGAT28400.071.56794
AGTAAGG12750.071.419751
ATAGGGC15100.071.206113
AGGGAAT24300.070.7789155
GGTAAGG13750.070.343241
ACGGGTA2150.070.189294
TAGGGCA9750.069.6493764
AAGGGAT39600.069.5467764
AGAGGGA51650.069.4822163
TAGAGGG35550.068.8473362
CGAGGGA13850.068.439313
AAGAGGG77050.067.509082
TAAGGGA20900.067.465893
Produced by FastQC (version 0.11.5)