FastQCFastQC Report
Mon 27 Feb 2023
SRR3128897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205139
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149900.6797757420280536No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC51170.23204886403986325Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG49170.22297914099746094No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG46110.20910246474258537No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC22940.10402972329635456No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22160.10049253130981765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG5850.081.1861342
AGGGATG41800.080.162915
CGTTTTT59000.076.731651
GTAGGGT7900.072.575993
GGTAAGG12100.071.951171
AGGGCAT20100.071.7798545
TAGGGCA10250.071.525524
AGGGTAC10350.071.288515
TAAGGGA18950.070.92793
GAGGGAT31300.070.869264
ATAGGGC14700.070.334043
AAGGGAT39950.070.111574
TAGCGGG11950.070.04362
ACGGGTA2350.069.993944
AGTAGGG33200.069.968932
AGGGAAT23200.069.683625
ATAAGGG29600.069.423432
GTAGGGC10150.068.0630653
GAATAGG14400.067.975491
TAGGGTA6600.067.645664
Produced by FastQC (version 0.11.5)