FastQCFastQC Report
Mon 27 Feb 2023
SRR3128896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278836
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155400.6819270890928527No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC54940.2410879940460832Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG51940.22792337842653002No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG48970.21489040896317244No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC23330.10237682746805825No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.10211353515566718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG44000.079.574395
CGTTTTT59050.075.126041
CGTAGGG5900.073.284992
TAGGGCA9950.073.214544
TAAGGGA18850.072.5555043
AGGGTAC11550.072.02135
GGTAAGG13600.071.602831
ATAGGGC15500.070.953353
GTAGGGT7900.070.795983
AGTAGGG36150.070.724492
GAGGGAT32450.070.534734
AAGGGAT41800.070.048774
TAGGGTA7300.069.532784
AGGGAAT24200.068.94185
GAGGGTA12250.068.292444
GGGCGAT10000.068.145586
CGAGGGA14300.068.033543
AGGGCAT19000.068.02195
AAGAGGG77100.067.723422
ACGGGAT7350.067.141444
Produced by FastQC (version 0.11.5)