FastQCFastQC Report
Mon 27 Feb 2023
SRR3128895.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128895.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100196
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234401.1160863081350503No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG22440.1068471704545671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84900.082.6004641
AGGGATG34200.071.450335
GAATAGG13700.070.960731
ACGGGTA1400.070.74624
CGTAGGG5900.070.3465042
ATAGCGG5250.070.114311
TAGAGGG39150.069.5113752
AGTAAGG14950.069.44691
ATAGGGC10600.068.9664153
AGTAGGG37200.068.337282
GTAGGGA15500.067.2469253
ATAGGGA17800.066.77173
GAGGGAT27950.066.48544
AAGAGGG81250.066.40712
CGAAGGG21450.066.403582
GAGGGTA12300.066.336554
AATGCGG4650.065.9677661
TAAGAGG14600.065.9399641
GATAGGG32100.065.824092
GGTAAGG13250.064.822671
Produced by FastQC (version 0.11.5)