FastQCFastQC Report
Mon 27 Feb 2023
SRR3128894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152977
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232781.0812005887661598No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG23170.10761842787916451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86650.082.586631
CGTAGGG5950.075.0763852
GAATAGG15550.074.430991
AGGGATG31550.072.0992665
TAGGGCA10800.071.368244
AGTAGGG37100.070.215432
ATAGGGC11150.069.9713
GTAGGGC10450.068.811343
AGTAAGG13550.068.750361
AGGGTAT10950.068.244545
TAGAGGG40650.067.785032
GAGGGAT25150.067.4612054
AATAGGG34650.066.630792
ATAGGGA17700.066.38223
TAGGGTA6950.065.595174
GTACGGG5450.065.5713042
AGGGCAT16150.065.477925
AAGGGAT36500.065.411744
TAAGAGG16450.065.210571
ACGGGAT4650.064.686244
Produced by FastQC (version 0.11.5)