FastQCFastQC Report
Mon 27 Feb 2023
SRR3128893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268459
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237781.0482005625845563No Hit
CCTGTCTCTTATACACATCTGACGCAAGATGGCTCGTATGCCGTCTTCTG24430.1076942541170019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86950.083.016681
TAGGGCA10100.076.3108144
ATAGGGC10350.074.01353
GTAGGGC10600.073.597973
AGGGATG36100.073.033035
GAATAGG18200.071.358761
TAGAGGG44450.068.300582
GAGGGAT25700.068.025774
AATAGGG36500.067.854932
CGAAGGG22550.067.7330252
AGTAGGG41250.067.674672
CGTAGGG6750.067.5354162
ATAGGGA19050.066.6089253
ATAGAGG18350.066.159661
TAAGAGG15050.065.971381
GATAGGG34650.065.781252
GTAGGGA15950.065.706423
TATAGGG17650.065.235662
GGTAAGG14500.065.2285461
ACGGGAT5750.064.5688254
Produced by FastQC (version 0.11.5)