FastQCFastQC Report
Mon 27 Feb 2023
SRR3128892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391420
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32990.8428286750804762No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC29370.7503448980634613No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG25970.6634816820806295No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG25420.6494302794951714No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15830.4044249144141842No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT7830.2000408768075213No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT7810.19952991671350465No Hit
GAACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT7230.1847120739870216No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG6490.1658065505084053No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT6390.163251750038322No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT5860.14971130754688058No Hit
GGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT5490.14025854580757244No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT5480.1400030657605641No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT5070.12952838383322263No Hit
GGAACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4530.11573246129477288No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4270.10908998007255633No Hit
ATAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4210.10755709979050636No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG4150.10602421950845638No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4120.1052577793674314No Hit
AGATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4110.10500229932042307No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT4000.10219201880333145No Hit
AGACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT3950.10091461856828982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGG156.808096E-494.313421
TCACGGG301.2991222E-894.2530062
AATGCGG1000.089.5977551
TAGCGGG900.089.016732
AGGGTAC1650.088.291865
GGGCGAT700.087.274696
GGACCGT600.086.155787
CGAGGGA2000.084.8277053
AGGGATG7800.084.348315
GTAGCGG452.7284841E-1183.834151
GACGGGT452.7284841E-1183.780453
AGTAAGG3100.083.665141
CGTTTTT13100.083.5141751
TAACGGG850.083.164422
GAGGGTA2000.082.471384
AGGGCAT4050.082.384655
GACCGTA401.035005E-982.239618
GGGTACG401.035005E-982.239616
AGAGGGT3350.081.5921553
GGATGGC5300.081.574597
Produced by FastQC (version 0.11.5)