FastQCFastQC Report
Mon 27 Feb 2023
SRR3128891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413296
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.7810382873291781No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC32150.7778928419341102No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG28760.6958693043242615No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG26120.6319925670705742No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16450.39801982114513573No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT8350.2020343772985947No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT8120.19646935852270525No Hit
GAACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT7690.18606519298517285No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG7530.18219387557585845No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT7420.17953234485695482No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT6520.15775618442956138No Hit
GGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT6220.1504974642870969No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT5530.1338024079594286No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT5430.13138283457860708No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG4820.116623436955596No Hit
ATAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4760.1151716929271031No Hit
GGAACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4670.11299407688436375No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4630.11202624753203516No Hit
AGATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4580.1108164608416244No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT4280.10355774069915992No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4180.10113816731833843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAGG650.094.10091
ACGGGTA550.093.998434
CGGGACG156.899518E-493.998435
TGCGGAC201.8366503E-593.998429
AGGGCAT4200.087.284255
AATGCGG650.086.8623661
TTGCGGG600.086.206952
CGGGTAA550.085.453125
AAGGGCA5200.084.960124
GGATGGC5500.084.598587
CGTTTTT12200.083.688091
CGGGAAT1300.083.152465
AGGGTAC1650.082.604685
AGTAAGG2800.082.338291
CGCTACG3150.082.0621263
GGGCGAT1050.080.5700766
TGATAGG1650.079.8431851
GATAAGG3300.079.8431851
AACGGGA1650.079.756253
GGGTACG650.079.537136
Produced by FastQC (version 0.11.5)