FastQCFastQC Report
Mon 27 Feb 2023
SRR3128890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429814
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32240.7500919002173032No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC30770.7158910598537972No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG29650.6898332767196974No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG28140.6547018012442591No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16070.37388265621873645No Hit
AGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT8980.208927582628765No Hit
GAACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT8710.20264579562322307No Hit
AAAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT8340.1940374208378508No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT7760.18054321171483478No Hit
ACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG7330.1705388842615643No Hit
AGAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT6890.16030189803031078No Hit
GGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT6200.1442484423494814No Hit
ACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT5570.12959093933655022No Hit
TAGACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT5420.1261010576668047No Hit
TCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG5180.1205172469952119No Hit
GGAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT5100.11865597677134761No Hit
ATAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4910.11423545998966995No Hit
GGAACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4710.10958228443000925No Hit
GCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT4610.10725569665017891No Hit
GAAGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4580.1065577203162298No Hit
AGATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT4480.10423113253639946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGG156.8817125E-494.0601961
TAGTCGG156.8817125E-494.0601961
TCACGGG254.9176197E-793.994542
GTAGGGT500.093.994543
GGTATAG201.8381736E-593.98367
GGATGGC6350.086.583317
CGTAGGG600.086.161662
TAGGGTC600.086.161664
ATAGCGG1150.085.881041
AGGGTAG1150.085.811115
CGTTTTT14100.085.387981
AGGGCAT3800.085.327215
GACGGGT500.084.595083
AAGGGAC2750.083.7405854
AGGGATG7750.083.675725
AATGCGG900.083.609061
GTAGCGG800.082.302671
AGGGCTA1550.081.856685
ACGGGTA750.081.461934
GATGGCA6150.080.9939968
Produced by FastQC (version 0.11.5)