FastQCFastQC Report
Mon 27 Feb 2023
SRR3128889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2277898
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283721.2455342600941746No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG29740.13055896269279837TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC24830.10900400281311981TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102700.082.857651
CGTAGGG6850.075.03252
TAGGGCA11800.071.928994
AGTAGGG36450.071.92682
ATAGGGC13350.071.701513
ACGGGTA2250.071.25414
TAGGGTA7750.070.578074
AGGGATG27950.070.2793665
ATAGGGT10550.070.1714863
GTAGGGT7850.069.6789863
TAGAGGG40050.069.2290342
AAGAGGG87200.066.998992
ATAGAGG15350.066.990721
AATAGGG35400.066.734122
GAGGGAT24150.066.581064
ATGTAGG7450.066.4813161
GAATAGG13950.066.27481
AGGGTAC12650.066.1245655
ATAAGGG33400.065.930122
AGGGCAT18550.065.61295
Produced by FastQC (version 0.11.5)