FastQCFastQC Report
Mon 27 Feb 2023
SRR3128888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2309185
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273831.1858296325326902No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG30130.13047893520874249TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC27680.1198691313168932TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104200.082.870561
TAGGGCA11000.075.196354
ATAGGGC13850.073.2960743
TAGGGTA7200.072.454814
AGTAGGG36900.070.857952
AGGGTAC11100.070.7082755
GAATAGG15400.069.670041
AGGGATG28600.068.688975
TAGACGG3250.068.0528641
GTAGGGC9900.067.885593
TAGAGGG42000.067.516232
AATAGGG33600.067.460242
ATAGAGG15700.066.840111
GGTAAGG14900.066.638951
CGTAGGG7150.066.428662
AGGGAAT16350.066.4004445
ATAGGGA16450.066.282493
AAGGGGC80850.066.2676544
CGAAGGG24200.066.069912
GTAGGGT8500.065.796813
Produced by FastQC (version 0.11.5)