FastQCFastQC Report
Mon 27 Feb 2023
SRR3128887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395707
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283371.1828241099600243No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG31940.1333218127258467TruSeq Adapter, Index 16 (100% over 22bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC27010.11274333630949027TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104050.084.3043751
TAGGGCA12700.076.975514
ATAGGGC13900.073.712943
AGTAGGG42450.073.516242
TAGGGTA8500.070.7756654
CGTAGGG7250.070.0132
GGTAAGG13850.069.314911
TAGTAGG11550.069.264891
GAATAGG15000.069.020431
AGGGTAC12850.068.39045
TAGAGGG42050.067.956422
AGGGATG28900.067.647565
ATAAGGG36850.067.342522
TAAGGGA17850.066.616893
GTAGGGT8700.066.4489063
AAGAGGG93600.066.431942
ATGAGGG37400.066.352192
GTAAGGG37400.066.2265242
ATGTAGG7200.066.013861
AATAGGG36950.065.76112
Produced by FastQC (version 0.11.5)