FastQCFastQC Report
Mon 27 Feb 2023
SRR3128886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252609
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196750.8734316519200624No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC27780.12332366602459638No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG24440.10849641460191273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71900.079.832831
ATAGAGG15400.073.810161
AGTAAGG13650.072.561541
GGTAAGG12800.071.484461
GTAGGGC12150.069.842963
AGGGTAC11600.069.679735
TAGGGCA11650.069.603154
ATAGGGC13200.069.2873843
AGGGCAT19200.069.2661745
ATAGGGA18250.069.230633
GTACGGG5850.068.499822
AGTAGGG40050.068.273452
AGACGGG15000.068.201652
AAGAGGG84350.067.5162352
TAGGGTA5600.067.3485644
AGGGATG30650.067.3085255
TAGAGGG41000.066.806492
GAGGGTA13550.066.801824
TAGGGTC7000.066.675084
CGTAGGG5950.066.556232
Produced by FastQC (version 0.11.5)