FastQCFastQC Report
Mon 27 Feb 2023
SRR3128885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280301
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189580.8313814711303463No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC30910.13555228015950527No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG25480.11173963437283062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT73600.080.5508351
AGACGGG15450.070.61992
ATAGGGA19450.069.83293
ATAGAGG15850.069.761511
TAGGGCA9750.069.412824
CGTAGGG6100.069.3873442
AGTAAGG13850.069.303911
GTAGGGC12300.068.777953
GGTAAGG13700.068.688931
AGTAGGG39850.068.331022
TAAGGGA17200.068.311443
TAGAGGG42550.068.1951142
AAGAGGG88000.067.657922
AGATAGG14500.066.5216751
CGAAGGG25100.066.515412
AGAGGGC33250.066.433643
AGGGCAT21800.066.401225
AGGGTAC12050.066.304615
ACGGGTA3100.065.191154
GATAGGG36300.065.037632
Produced by FastQC (version 0.11.5)