FastQCFastQC Report
Mon 27 Feb 2023
SRR3128884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2422003
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193510.7989668055737339No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC29860.12328638734138644No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG26000.10734916513315632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76050.080.6087651
AGTAAGG15050.073.7755661
TAGGGCA11250.073.525794
ATAGGGA20850.072.356673
ATAGGGC13600.070.8426063
ATAGAGG18400.069.8042451
AGTAGGG41450.069.618292
GTAGGGT9100.068.689413
AGGGATG36450.068.5951845
AAGGGAT37300.068.544024
AGATAGG14950.067.975061
GTAGGGC12000.067.755483
AGGGTAC13300.067.493395
TAAGGGA19050.067.3514943
TAGACGG3400.066.4200741
GATAGGG36650.066.297342
AGGGATT21850.066.2489245
TAGTAGG10450.066.181731
AGGGCAT18500.066.051275
ATAAGGG33250.065.5852052
Produced by FastQC (version 0.11.5)