FastQCFastQC Report
Mon 27 Feb 2023
SRR3128881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124987
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150900.7101219913345352No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30470.1433891124981No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC30060.1414596889298617No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG28180.13261257598281778No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG24050.11317716296617344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54100.080.254481
ACGGGTA1600.073.433864
AGGGATG39850.073.23685
AGTACGG3700.072.466611
ATAGGGC12400.070.875523
ACGGGAT7550.070.340884
TAAGGGA15650.069.370363
CGTAGGG6800.069.110972
GAATAGG13350.068.357371
TAGGGAT17150.067.687614
TAGGGCA10650.067.5177844
AGGGAAT21450.067.263155
AGGGCAT19450.066.930845
AGTAAGG16300.066.663671
AAGGGGC66600.066.474184
ATAGGGA22150.066.4120563
AGTAGGG35000.066.3307342
AAGGGAT32650.066.214184
TAGAGGG36900.065.844592
GGTAAGG11450.065.7323761
Produced by FastQC (version 0.11.5)