FastQCFastQC Report
Mon 27 Feb 2023
SRR3128880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760342
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226480.8204780422136096No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG37560.13607009566205927TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC36920.1337515423813426TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG28560.10346544015198117TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87450.080.12781
CGTAGGG5800.074.005522
ATAGGGA23750.073.880543
AGGGATG42650.073.821515
GTAGGGT8900.072.0774843
GAATAGG17000.071.8898241
TAGGGTC8200.070.752534
AGTAGGG46750.070.72732
ATAGGGC15450.069.302373
GGTAAGG18600.069.2574161
GAGGGAT35400.069.15364
TAGAGGG48550.069.076622
AGACGGG18600.068.977382
AAGAGGG102650.068.558392
AAGGGAT43450.068.39144
TAAGGGA21000.068.281813
ACGGGTA3000.067.607974
AGATAGG16450.067.40911
TAGGGCA10650.067.320094
AGGGTAT15350.066.738325
Produced by FastQC (version 0.11.5)