FastQCFastQC Report
Mon 27 Feb 2023
SRR3128879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2844443
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219800.7727347674043741No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG40740.14322663523227572TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC38940.1368985070187731TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG30200.10617192891543266TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84100.080.456241
CGTAGGG8550.075.359382
GTAGGGT8950.074.03993
AGTAGGG48250.073.8847352
ATAGGGC14200.072.150263
AGGGATG44650.071.4690555
TATAGGG22200.070.3344352
GAATAGG16500.070.153291
ATAGGGA22550.069.81813
ATAGCGG6200.069.063171
TAGGGCA12500.068.803544
TAGGGTC8950.068.788854
TAGGGAT18800.068.245584
TAGAGGG51000.067.41092
ATAGAGG19500.067.323461
GTAGGGA22200.067.108283
AAGGGGC92250.066.891074
ATAAGGG42500.066.617822
ATAGGGT10600.066.505133
AGTAAGG18400.066.4893951
Produced by FastQC (version 0.11.5)