FastQCFastQC Report
Mon 27 Feb 2023
SRR3128877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170193
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282991.303985405906295No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28830.13284532758146397No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG25110.11570399499030731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111200.084.187191
AGGGATG39300.072.70225
AGATAGG11700.072.153511
ATAGGGA18950.072.1453553
ATAGGGC12700.070.900493
GGTAAGG13750.070.656941
GAATAGG13250.070.119791
TGTAGGG16450.069.353612
TAGGGCA8850.069.2499854
TAGAGGG36950.067.876082
GATAGGG31800.067.453412
ACGGGTA2450.067.34754
CGTAGGG5050.067.214142
AGTAGGG33950.067.0697862
GAGGGAT29450.066.752934
TAAGGGA17800.066.477293
ATAGAGG14350.066.3880161
TATAGGG17500.066.269952
ACTAGGG10200.066.0929952
GTAGGGC8350.066.057023
Produced by FastQC (version 0.11.5)