FastQCFastQC Report
Mon 27 Feb 2023
SRR3128876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228529
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT277561.2454852505845784No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30000.13461794753400114No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG27600.12384851173128104No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC23120.10374556489953687Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107300.085.1669851
ATAGGGC12250.076.730793
GAATAGG14000.075.963011
AGGGATG37400.074.014955
CGTAGGG7300.071.507042
AGTACGG2800.070.58511
TAGGGCA10600.070.4964144
ATAGGGA18850.069.3121263
TAGAGGG37000.069.269722
GGTAAGG13200.068.446161
GAGGGAT26400.068.360154
AGTAGGG34650.068.267412
GTAGGGC9500.067.77553
ATAGAGG15250.066.959421
TATAGGG15550.066.231172
AAGGGAT43600.066.076924
AGGGAAT21150.065.996655
GATAGGG32500.065.838022
AAGAGGG82050.065.4542
CTACGAC7750.064.8870245
Produced by FastQC (version 0.11.5)