FastQCFastQC Report
Mon 27 Feb 2023
SRR3128875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364521
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285841.20887063384085No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30570.12928622752768953No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG28060.11867096972283181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT109000.083.918221
AGTACGG2900.073.013141
AGGGATG38650.071.983825
ATAGGGC12750.071.878053
TAGGGCA11850.070.991534
AGTAAGG15050.070.6575241
GAATAGG14100.069.7450941
TAGGGTA5750.068.656764
AAGGGAT42350.068.581494
AGATAGG13200.068.440591
TATAGGG17750.068.309962
GTAGGGC11300.068.2083053
AATGCGG4850.067.9114151
TGTAGGG16950.067.652342
AGTAGGG38900.067.534372
ACTAGGG10300.067.528512
TAAGGGA20900.067.460083
TAGAGGG42850.067.34112
GAGGGAT29300.067.207584
CGTAGGG6200.066.704272
Produced by FastQC (version 0.11.5)