FastQCFastQC Report
Mon 27 Feb 2023
SRR3128873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3002701
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217840.7254801593631867No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG44640.14866615090879845Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC41890.13950772987387022TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG32560.10843570505355012Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95750.077.992751
ATAGGGA28900.072.691413
AGGGTAC15500.072.466895
CGTAGGG8250.070.678462
AGATAGG17400.070.5841751
ATAGGGC18000.070.4960253
ATAGCGG6900.070.2431951
AGGGATG45000.069.764965
TAAGGGA25400.069.570883
AGTAGGG50300.069.367382
GAATAGG20800.069.226791
TAGGGCA13850.069.223534
AAGGGTA22050.069.0573354
AAGGGAT47000.068.196164
GGTAAGG18900.067.720971
TATAGGG25500.067.677642
GTAGGGC12450.067.5704963
TAGAGGG57850.066.97972
AGAGGGC43500.066.768653
AGTAAGG19500.066.361191
Produced by FastQC (version 0.11.5)