FastQCFastQC Report
Mon 27 Feb 2023
SRR3128872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124145
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225060.720389098457338No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG44910.1437513303639876Illumina PCR Primer Index 7 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC44340.14192683118101113TruSeq Adapter, Index 23 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG35380.11324698437492497Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96200.078.782541
ATAGGGC20050.071.723993
AGGGATG42250.070.518985
AAGGGTA22800.070.081194
ATAGAGG20800.069.888321
CGTAGGG7350.069.693072
AGGGAAT29950.069.1966865
TAAGGGA26850.068.961923
AAGGGAT47300.068.5559844
GAATAGG21450.068.209141
AGTAGGG48200.067.7623142
ATAGGGA30400.067.710793
ATAGCGG7300.067.666841
TAGGGCA14050.067.566664
TAGAGGG61950.067.287252
ATAAGGG46150.066.800942
AGAGGGC46000.066.7133563
ATAACGG2900.066.5112841
GTAGGGC11050.066.346763
AGATAGG17750.066.259951
Produced by FastQC (version 0.11.5)