FastQCFastQC Report
Mon 27 Feb 2023
SRR3128871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385991
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273361.1456874732553475No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG29180.12229719223584665No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC25940.10871792894440926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT95450.083.3894651
ATAGGGC14350.073.615153
AGGGATG36250.072.8548665
TAGGGCA11700.070.941094
TAGAGGG46550.069.39722
ATAGCGG4850.069.069871
GGTAAGG14800.068.221951
TAAGGGA19550.068.0257643
ATAGGGA18100.067.743223
AAGGGAT39700.067.591624
TAGGGTA7400.067.553094
AGTAGGG39050.067.2674942
AATAGGG38750.066.9363562
CGTAGGG7550.066.835612
ATAGAGG17800.066.5312651
GTAGGGA19100.066.418643
TAGGGAT15550.066.417854
TGTAGGG17650.066.264072
AAGGGTA18250.065.894394
ACGGGTA2800.065.686744
Produced by FastQC (version 0.11.5)