FastQCFastQC Report
Mon 27 Feb 2023
SRR3128870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2437988
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269281.104517331504503No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG30460.12493908911774793No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC28200.11566915013527547No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97650.083.198761
ATAGGGC12800.073.431433
AGGGATG35650.073.031835
ATAGGGA20000.070.494173
GGTAAGG17200.070.0583341
AAGGGAT39250.069.087284
AGTAGGG43850.068.752582
TAAGGGA18550.068.6573943
TAGAGGG44800.068.23952
TATAGGG19750.068.108212
GTACGGG5350.067.6920242
TAGGGCA12200.067.0272454
ATAGAGG15850.066.8192441
TAGGGAT16300.066.601854
GTAGGGC11900.066.347463
AGGGAAT20200.066.30645
GAATAGG14650.066.187791
GCATAGG5550.066.153061
GTAGGGA18350.066.076283
GAGGGAT30350.065.65524
Produced by FastQC (version 0.11.5)