FastQCFastQC Report
Mon 27 Feb 2023
SRR3128869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568871
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276911.0779443576575078No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG31690.12336158569270314No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC27920.10868587795961727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102450.084.055781
ACGGGTA2650.072.712344
AGGGATG36400.071.9115455
ATAGGGC14050.070.5791243
TAGGGTA7300.069.529814
AAGGGAT42300.068.773394
GGTAAGG16300.068.420971
GAGGGAT29800.068.129884
TAGAGGG47850.068.064622
ATAGCGG6650.067.932571
AGTAGGG43400.067.463442
TAAGGGA19950.067.3741463
AGGGAAT24400.066.44685
TAGGGCA11550.065.917884
GTAGGGC10700.065.8836443
GTAGGGA19350.065.820093
CGTAGGG7200.065.273612
AAGAGGG93450.064.7748952
AGTAAGG16650.064.721671
ATAGGGA19350.064.60573
Produced by FastQC (version 0.11.5)