FastQCFastQC Report
Mon 27 Feb 2023
SRR3128868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144776
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416271.9408553620517948No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG46510.2168524824970067No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT37320.17400418505242504No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC36200.16878219450422793No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT32220.15022547809188463No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT27330.1274258943591312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT159950.086.957381
AGGGATG37750.074.689165
TAGGGTA6250.070.947124
ACGGGTA1950.070.1536564
AAGGGAT37550.069.7220154
AGTAGGG34650.069.703352
GTAGGGT8200.069.607893
GTACGGG4700.069.252932
TAGGGCA9650.068.9253164
GAATAGG12300.068.6844561
TAGAGGG38300.068.3567052
ATAGGGC12150.068.331873
TAGGGTC6100.068.051834
AAGGGTA14850.067.978924
AGGGTAC9700.067.823415
ATAGAGG14700.067.744771
GAGGGAT26900.067.514244
AGTAAGG14150.067.376041
TAGGGAT13550.067.190054
AGGGCAC15100.066.909045
Produced by FastQC (version 0.11.5)