FastQCFastQC Report
Mon 27 Feb 2023
SRR3128866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248557
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423851.8849866825701995No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG51770.23023654726119905No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC40090.17829212245898146No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT39870.17731371719729586No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT34430.15312042345379726No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT29250.13008342683774526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT160000.086.62961
ATAGGGC12500.075.1952443
AGGGATG41550.073.86225
TAGGGCA9400.070.9955144
GTAGGGA17650.069.230753
AAGGGAT36800.068.707624
ACGGGTA3150.068.6305854
AGTAGGG35300.067.7647552
GGTAAGG11150.067.514751
ATAGGGA20450.067.3355943
TAGGGAT17450.067.061674
CGTAGGG6700.065.934672
GAGGGAT29900.065.544364
AAGAGGG81900.065.4156652
TAGAGGG39100.065.2655262
AGTAAGG13700.065.2509161
AGAGGGC32550.065.117243
AGAGGGA51850.064.989143
GAATAGG11950.064.9635241
AAGGGTA14550.064.600734
Produced by FastQC (version 0.11.5)