FastQCFastQC Report
Mon 27 Feb 2023
SRR3128865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656902
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54240.8256939391263842No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG37420.569643569360422No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC36760.5595964085967161TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG35660.5428511406572061No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28680.4365948040955881No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT12920.19668078343497206No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT11950.18191450170649504No Hit
TAGACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT10480.159536734550968No Hit
GAACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT9160.13944241302355603No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT9070.13807234564668702No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG9050.13776788622960504No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT8260.12574173925486604No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT7810.11889140237052102No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT7230.11006207927514301No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG6990.10640856627015902No Hit
GAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT6920.10534295831037203No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT156.904196E-493.9886557
CGTAGGG1450.087.753822
AGGGTAC3600.084.8508765
AGGGATG23000.083.363855
CGTTTTT22400.083.320251
CGCTACG5150.083.273043
ATAGCGG1250.082.949941
CGGGAAT2000.082.240085
TCCGCTA5350.081.928051
AGGGCAT4100.081.3804245
ATGGCAC16050.081.105489
GGATGGC17950.080.8983157
CAGGGAT5900.080.6751254
GATGGCA16300.080.438158
GGAATGC8850.079.6514057
TAGCGGG2900.079.628472
AATGCGG950.079.377941
GATGACC12100.078.841728
ACGGGTA1200.078.545084
GATTCGC301.4564885E-678.323888
Produced by FastQC (version 0.11.5)