FastQCFastQC Report
Mon 27 Feb 2023
SRR3128864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677986
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.7899868138870124No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC38710.5709557424489592TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG38680.570513255435953No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG38090.5618110108468317No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30090.4438144740451868No Hit
AAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT13380.19734920780075105No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT12960.19115438961866468No Hit
TAGACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT10790.15914782901121852No Hit
AGAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT9650.14233332251698413No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG9200.1356960173218916No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT9020.1330410952438546No Hit
GAACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT9010.13289359957285252No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT8340.12301138961571477No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT7590.11194921429056057No Hit
GAAGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT7320.10796683117350506No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG7210.10634437879248244No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT156.9015624E-493.998027
ATAGCGG1050.089.607831
TAGCGGG2800.085.662192
AGGGATG20950.085.0244455
TAGGGCG950.084.1034854
CGTAGGG1350.083.609292
CGTTTTT22550.083.2399141
GATGACC11350.081.575378
ACGGGTA1850.081.295584
AAGGGAT15900.080.9924
CGCTACG5400.080.942733
CGGGAAT1800.080.9427265
GGATGAC11850.080.9096767
GGGATGA13800.080.034536
GTAGGGC3350.079.968453
CACGGGT1000.079.898313
TCCGCTA5650.079.100711
TAAGGGA5750.078.46793
CGAAGGG4450.077.1506962
AGGGCAT5850.077.126575
Produced by FastQC (version 0.11.5)