FastQCFastQC Report
Mon 27 Feb 2023
SRR3128862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1549635
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94440.6094338344190728No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG22840.14738954657064404No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC21650.1397103188815431No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG19780.1276429610843844No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.10331465151471153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41150.079.300661
CGTAGGG5300.078.264452
AGGGATG25150.075.117415
AGTAGGG27300.072.34512
GTAGGGA12750.072.091193
AGGGCAT11700.071.496815
AGGGTAA5200.071.39645
ATAGGGC8500.070.982093
GTAGGGC6300.070.330723
ACGGGTA2150.070.1567154
TACGGGT1550.069.944563
TAGGGAT10050.069.884034
TAGGGCA8400.069.582524
AAGGGTA10050.069.415014
TAGGGTA4550.068.3738864
ACTAGGG7950.068.1849442
GGTAAGG9750.067.228071
ATAGGGA13100.066.566883
AGGGTAT11300.065.709965
TAAGGGA15300.065.621473
Produced by FastQC (version 0.11.5)