FastQCFastQC Report
Mon 27 Feb 2023
SRR3128861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574473
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93520.5939765242084177No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG24350.15465492263125502No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC22170.1408090199069784No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG20600.13083742941288926No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16030.10181184434410752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41600.078.947931
AGGGATG27000.071.193675
TAGGGAT10850.070.172534
TAGGGCA8900.069.177234
AGTAGGG25250.068.921282
GTACGGG2800.068.871382
GTAGGGA13450.068.488183
TAGAGGG26700.067.820732
ATAGGGC8600.067.218453
AGATAGG7850.067.13161
TAAGCGG4100.066.5614551
AGGGCAT13350.066.184835
AAGGGTA10100.064.680794
AAGGGCG7450.064.346634
CGGGAAT2850.064.313425
ACTAGGG8150.064.058742
ATAGGGA11700.063.8694463
GATAGGG21450.063.8085252
AGGGTAA6050.063.7001345
AAGAGGG58350.063.5988352
Produced by FastQC (version 0.11.5)