FastQCFastQC Report
Mon 27 Feb 2023
SRR3128860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1652424
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.5835669295531898No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG24820.1502035797107764No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC23260.14076290346787507No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG22020.13325877619787657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGG4850.083.328762
CGTTTTT41900.076.328731
ACGGGTA2150.072.1318054
GTAGGGA10800.071.797853
GGTAAGG9400.070.547951
TGTAGGG11800.070.092062
AGGGATG25500.070.031715
TAGGGCA8000.069.905014
AGTAGGG24750.069.873222
ATGCGGG7400.067.950122
ATAGGGC9050.067.506583
GTAGGGT6550.067.443153
AGTAAGG10150.067.188521
TAAGGGA13600.066.691383
GAATAGG9750.066.568311
ATGTAGG5350.065.932661
AGGGCAA16200.065.850975
ACTAGGG7600.065.543622
ATAGGGA14750.065.3150253
AGTACGG1950.065.1211851
Produced by FastQC (version 0.11.5)