FastQCFastQC Report
Mon 27 Feb 2023
SRR3128859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376327
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC37550.997802443088059TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG33300.8848687444695703No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG29370.7804382890411796No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.7296845562502824No Hit
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT8960.23809080932274324No Hit
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG8260.21948996484440395TruSeq Adapter, Index 21 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT8120.21576979594873608No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT8040.2136439851512116No Hit
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT7220.19185442447658552No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT6300.16740760030505386No Hit
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT5980.15890435711495587No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT5900.1567785463174314No Hit
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT5840.155184188219288No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG5840.155184188219288No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC5600.14880675582671452No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4960.1318002694465186No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCTATAGGACTCGT4810.12781437420116018No Hit
AGATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4490.11931113101106218No Hit
ATAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4440.11798249926260938No Hit
AAGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4310.11452805671663206No Hit
AGGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4310.11452805671663206No Hit
AATGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4220.11213651956941703No Hit
GGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4190.11133934052034533No Hit
AAGACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4130.10974498242220197No Hit
AGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4070.1081506243240586No Hit
GCAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT3910.1038990027290096No Hit
GAACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT3900.10363327637931904No Hit
AGGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT3850.10230464463086625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG301.29584805E-894.286454
GTACACG156.902724E-493.985788
AGTAAGG2450.088.5610351
AGAGGGC4650.087.189623
AGGGTAT2650.085.11925
AGGGCGA1550.084.890395
CGGGTAT500.084.5872045
GGGCGAT1000.084.5872046
AGGGATG6200.084.132445
TAGAGGG5100.084.118312
GGGTATC2550.082.9286356
CGTAGGG1150.081.988232
GGATAGG1900.081.924031
TACGAGG750.081.758531
GTACAGG1050.080.860081
AGATAGG2100.080.860081
GGGCTTA353.8615326E-880.559246
ACGGGTA650.079.7808464
GCAGGGT1600.079.55423
CGAAGGG3200.079.55422
Produced by FastQC (version 0.11.5)