FastQCFastQC Report
Mon 27 Feb 2023
SRR3128858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394827
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC40281.0201936544359935TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG34330.8694947407345496No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG31420.7957915745377089No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.6868831158963293No Hit
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG10260.2598606478280361TruSeq Adapter, Index 21 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT9870.24998290390474817No Hit
AAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT9750.24694359808219804No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT8890.2251619063539221No Hit
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT7620.19299591973193322No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT6810.17248060542971985No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT6630.16792164669589466No Hit
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT6500.16462906538813202No Hit
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT6390.1618430350507944No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG5900.14943253627538136No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC5400.13676876201475582No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT5260.13322290522178068No Hit
AGATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT5000.12663774260625538No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCTATAGGACTCGT4960.12562464066540535No Hit
ATAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4800.1215722329020052No Hit
AAGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4730.1197993045055176No Hit
GGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4710.11929275353509258No Hit
AGGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4650.11777310062381752No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTATAGGAC4640.11751982513860501No Hit
AATGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4570.11574689674211742No Hit
AAGACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4430.1122010399491423No Hit
AGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4350.1101748360674422No Hit
GAACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4330.10966828509701718No Hit
GCAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4270.10814863218574211No Hit
AGGACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4090.10358967345191691No Hit
AGGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4080.10333639796670441No Hit
AGACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4050.10257657151106687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGC156.9005275E-493.994173
CAGGGTA750.087.72794
AGGGCGA1200.086.161325
AGGGTAC2350.085.994675
AATGCGG1150.085.951391
CGTAGGG1050.085.107022
GCATAGG1350.083.677551
AGGGAAT6900.083.09635
TAAGCGG850.083.062271
TAGGGTA1650.082.6009454
AGGGATG6550.082.513975
CGGGTCA401.033186E-982.2448965
TAGAGGG4800.081.32762
CGTTTTT12850.081.3171
GTACAGG1350.080.190991
AGGGCAA3050.080.126185
AGTAGGG5050.080.095512
CAAGGGT1350.080.069113
AGGGCGT950.079.152995
TGAAAGG2750.078.732961
Produced by FastQC (version 0.11.5)