FastQCFastQC Report
Mon 27 Feb 2023
SRR3128857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400830
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC42981.0722750293141732TruSeq Adapter, Index 21 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG37060.9245814934011924No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG32690.8155577177356984No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.6843300152184217No Hit
AGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT10600.264451263627972No Hit
ACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG10010.24973180650150936TruSeq Adapter, Index 21 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT9290.23176907916074144No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT8740.2180475513309882No Hit
GGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT8470.21131152857820024No Hit
AGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT7310.1823715789736297No Hit
GAAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT7190.17937779108350174No Hit
ACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT6290.15692438190754185No Hit
TCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG6230.15542748796247785No Hit
GGAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT6220.15517800563830053No Hit
GCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT6100.15218421774817256No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC5530.13796372527006462No Hit
GGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT5170.12898236159968066No Hit
ATAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT5120.127734949978794No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCTATAGGACTCGT5060.12623805603373No Hit
AAGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4900.12224633884689269No Hit
AGATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4730.11800513933587806No Hit
AGGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4700.11725669236334606No Hit
AAGACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4640.11575979841828207No Hit
AATGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4540.11326497517650874No Hit
AGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4470.11151859890726741No Hit
AGGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4460.11126911658309009No Hit
GCAGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4380.10927325798967143No Hit
AGGACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4340.1082753286929621No Hit
GAACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT4040.10079085896764214No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG254.8878974E-794.088291
GATGTAG156.900545E-493.9943858
ACGGGTA301.3242243E-893.9943854
CGATCAG254.917056E-793.9943859
AGGGTAC2650.088.673955
GCGGGAT1500.087.7280964
GGATACG650.086.764058
TAGGGTA1550.084.8981554
AGTAAGG1850.083.916571
AGGGATG7650.083.550575
TAGGGCG452.7284841E-1183.550564
GGGCGTA401.033186E-982.2450946
CGGGAAT800.082.2450945
CGTTCTG750.081.543181
AGGGTCC1100.081.176975
GGCGATC353.8597136E-880.566627
AGGGAAT8000.080.482695
CGTTTTT14400.080.367071
AGGGCGA2050.080.239115
AAGGGTA3600.079.6341254
Produced by FastQC (version 0.11.5)